This page covers Ozone IV Therapy including its many benefits. IV League provides mobile Ozone IV Therapy.

Ozone therapy has been utilized and heavily studied for more than a century. Ozone therapy is still a controversial form of alternative therapy because of the medical paradox it creates, how can a gas be both dangerous to health as a pollutant in the air, yet can also be used to effectively treat some of humanity’s most threatening diseases, however its effects are proven, consistent, safe and without side effects.

Ozone (O3) is an allotropic form of oxygen gas which is generally found in the uppermost layer of atmosphere, it acts as a protective layer to our environment. Ozone is produced in the upper atmosphere when UV light strikes the oxygen rising from plants, plankton, and algae in our forests and seas.

It then falls back through the atmosphere, as it is heavier than air, combining with pollutants and water, cleaning the air and forming peroxides that benefit plants.

The fear of infusing a gas into a vessel or ozone’s toxicity since we frequently hear about the unhealthy ozone levels in the atmosphere do not apply to properly administered medical ozone. The potential benefits of ozone therapy are profound and without associated detrimental effects.

Ozone iv therapy

Benefits of Ozone IV Therapy

Medical ozone has powerful oxidizing properties, forming oxygen-free radicals that leads to the destruction of microorganisms and improves stimulation of the process of healing. Ozone therapy is effective in managing many skin conditions, mainly wound healing, primarily because of its ability to promote inflammation in the body such as the secretion of immunomodulatory products and, reduction of the oxidative damage.

Ozone therapy has been widely studied in infected wounds, gangrene, burns and, circulatory disorders, showing to be highly effective in the outcome of these conditions. Its characteristics as an activator of the antioxidant system make this molecule capable of stimulating tissue healing and an anti-inflammatory environment at low doses, but when used in high concentrations, it can lead to an exacerbation of inflammation.

Although ozone therapy is now used all over the world, it has not yet been accepted as orthodox medicine in all countries. About two decades ago, both nitrogen oxide (NO) and carbon monoxide (CO) were only considered as toxic air pollutants or gases derived from cigarette smoke.

However, today, they are regarded to as essential gases, since both NO and CO are responsible for very important physiological actions within the body. Similarly, hydrogen sulfide, a toxic gas, is now used as a drug for the treatment of osteoporosis.

Thus, our understanding of toxic compounds and their effects within the body appears to be constantly changing as we have realized that toxicity depends entirely on the dosage.


Ozone as a chemical element was discovered at the end of the 18th  century. In 1785 Martinus Van Marum, a Dutch physicist, subjecting oxygen to electrical discharges noted some specific “odor of electrical matter”. In 1848 Christain Schonbein, a German physicist, having repeated the experiments, named the smelling gas Ozone (from the Greek “ozone”-odorant) and described some of its properties.

He thought it to belong to the same class of bromine and chlorine and to have negative electric charge. Mariniak and Delarive showed that it is an allotropic form of oxygen and Mulliken and Dewar clarified its molecular structure. In 1957 Warner created tubes for magnetic induction, capable to produce ozone in large quantities and that launched an extensive research of ozone properties.

Ozone was used for the first time to disinfect operating rooms in 1856 and subsequently for water treatment in 1860. The German Army used ozone to treat battle wounds and other infections during World War I.

After the turn of the century, interest began to focus on the uses of ozone in medical therapy. However, it was not until 1932 that ozone was seriously studied by the scientific community, when ozonated water was used as a disinfectant by Dr. E.A. Fisch, a German dentist.

One of his patients was the surgeon Erwin Payr, who immediately saw the therapeutic possibilities of ozone in medical therapy. Dr. Payr, along with the French physician P. Aubourg, was the first medical doctor to apply ozone gas through rectal insufflations to treat mucous colitis and Fistulae.

Medical ozone has been around for over 150 years. It has been used to treat infections, wounds, and multiple diseases, ozone’s effectiveness has been well documented. A text on medical ozone therapy was published by Dr. Charles J. Kenworth in 1885!

The best technology for producing ozone gas was designed and built by Nikola Tesla in the 1920’s. Heads of leading medical institutions in the U.S. contributed to a 1929 book “Ozone and Its Therapeutic Actions” describing the treatment of 114 diseases using ozone.

Today in Germany, and other countries, ozone therapy is commonplace. Over 7000 doctors in Germany use it daily. In fact, in Germany, ozone generators are in ambulances for treatment of stroke victims. The incidence of permanent paralysis in these patients is much less than that in similar patients where ozone is not used.

According to the Europe based Medical Society for Ozone and the National Centre for Scientific Research in Cuba, physicians are currently treating the following diseases with different forms of ozone: abscesses, acne, AIDS, allergies, anal fissures, arthritis, asthma, cancerous tumors, cerebral sclerosis, circulatory disturbances, cirrhosis of liver, constipation, corneal ulcers, cystitis, decubitus ulcers (bedsores), diarrhea, fungal diseases, gangrene, gastroduodenal ulcers, glaucoma, hepatitis, herpes simplex and zoster, hypercholesterolemia, osteomyelitis, Parkinson’s disease, Raynaud’s disease, rheumatoid arthritis, senile dementia, sepsis, sinusitis, spondylitis, thrombophlebitis, wound healing and others. This list is not exhaustive

Many physicians and clinics offering this form of therapy in Canada and the United States have been forced out of practice by various government agencies.

Yet, in the past decade this therapy has gained more prominence and credibility in other apart of the world. For the most part, ozone therapy is available in Mexico, some Caribbean countries like the Bahamas, Cuba and the Dominican Republic as well as in Europe, especially Germany and eastern Europe.

Ozone IV Therapy Uses

Ozone was first suggested as a potable water disinfectant in the nineteenth century because of its powerful ability to inactivate microorganisms and it has been later confirmed by other researchers that this substance, when used for limited periods, can effectively disinfect the water supply.

It has been considered an effective alternative antimicrobial agent to chlorine, even with adverse toxicological problems arising from its use in high concentrations. Due to its ability to oxidize substances and destroy microorganisms, ozone has been widely used to sterilize air, clean water and remove odors.

Ozone therapy has been used and studied extensively for many decades. It has been used to treat infections, wounds and various diseases. Ozone has wide medical applications due to its effective antimicrobial function, antioxidant defense and the anticipation in wound healing and in the control of bleeding.

The effects on immunoregulation and antioxidant defenses, as well as the effectiveness of ozone therapies, have been gradually recognized, although the mechanisms involved in various conditions are still unclear. Due to its role as an activator of antioxidant defenses, ozone is widely used for cosmetic-related services and general health services.

As an anticancer, antiviral, antibacterial, and antifungal agent, ozone is unsurpassed, especially since there are no appreciable side effects. Oxygen deficit is key to the development and progression of all disease processes.

Dr. Otto Warburg’s work, confirmed by others, shows that this deficit and subsequent toxin buildup is the fundamental cause of all degenerative disease, especially cancers. Antioxidants help the body to protect itself from excessive oxidative damage caused by multiple free radicals, many of which are inactivated by ozone.


Though many pharmacological investigational studies have been carried out to establish the mechanism by which ozone shows its pharmacological action, but still none of them has explained fully the pharmacological effects of ozone. Some diverse but interlinked steps of mechanisms have been proposed by different scientists as described

a) In bacteria:  damage to cell envelope through oxidation of phospholipids and lipoproteins.     b) In fungi: ozone inhibits cell growth at certain stages.                                                                           c) In viruses: ozone damages the viral capsule and renders the reproductive cycle by peroxidation of inner cell contents.

Ozone therapy causes an increase in R.B.C. glycolysis rate which leads to stimulation of 2,3 diphosphoglycerate as a result of which an increase in amount of oxygen reaching the tissues is observed. Ozone stimulates the Krebs’s cycle by enhancing the oxidative carboxylation of pyruvate and stimulating the production of ATP.

Ozone administered at a very narrow range of concentration of about 30 to 55μg/cc causes the greatest increase in the production of interferon and greatest output of tumor necrosis factor and interleukin-2. The production of interleukin-2 launches an entire cascade of subsequent immunological responses that results in transcription of cytokines such as IL-2, TNF-α, IL-6 etc. These products help in proper functioning of our immune system.

Ozone therapy has shown many positive results in the treatments of various diseases. But the main problem lies with the appropriate concentration of the ozone for curbing the activity of cytotoxic T-cells and increasing the amount and activity of the CD4+ T-regulatory cells.


Topical applications

O3 has been widely acclaimed as one of the best bactericidal, antiviral and antifungal agents and has also been used as a clinical therapeutic agent for chronic wounds, such as ulcers, ischemic ulcers and diabetic wounds. The beneficial effects of O3 on wound healing is accounted for by its ability to decrease the frequency of bacterial infection, ameliorate impaired dermal wound healing or increased oxygen tension by O3 exposure in the wound area.

Role of O3 has been reported to activate transcription factor NF-κB which is important to regulate inflammatory responses and eventually the entire process of wound healing.

Dental application

Periodontitis, a destructive inflammatory disease of the supporting tissues of the teeth occurs due to accumulation of specific microorganism or a group of microorganisms in oral cavity, resulting in progressive destruction of periodontal ligament and alveolar bone with periodontal pocket formation, gingival recession, or both. It has been estimated that more than 500 different bacterial species are capable of colonizing the adult mouth and the lesions of the oral cavity have an immense impact on the quality of life of patient with complex advance diseases.

Another use of ozone mostly used in children for treating primary carious lesions using a special device isolating the tooth which is treated with ozone-oxygen for about 60 sec. This procedure sterilizes the dental lesions and allows the re-mineralization of the tooth. The procedure was invented and propagated by prof. Edward Lynch and it was defined as “The revolution in Dentistry”.

Diabetes Mellitus

Ozone therapy appears to be an effective method for DM treatment. The reason is in ozone mechanisms when it can perform a number of processes, which provide its positive effect.

First, ozone improves the penetration of cellular membranes for glucose. It is achieved by stimulating pentose-phosphate pathway and aerobic glycolysis that in case of DM are inhibited. It promotes hyperglycemia decrease due to better transport of glucose into tissues.

In a study where a group of 70 patients with insulin-dependent and non-insulin-dependent diabetes were observed. After the course of ozone therapy, the average level of hyperglycemia had a 26% decrease. Positive effect was received in 92% of insulin-dependent cases and 89% of non-insulin dependent cases. It was expressed in decrease of hyperglycemia, thirst reduction, disappearance of polyuria, pruritus, weakness

Ozone therapy proved to be effective in the treatment of complicated forms of diabetes mellitus. In patients with diabetic foot syndrome ozone therapy, combined with topical treatment of purulent necrotic focus and with total metabolic effect, allowed to half-shorten the period for wound detersion from pyo-necrotic mass and development of regenerative processes, to lessen the number of limb amputations and of fatal outcomes, to decrease invalidism.

Treatment of cancer

When cancer cells get exposed to ozone gas therapy, they die rapidly.  Ozone  find  its  application  in  fighting  against cancer  by  variety  of  mechanisms,  such  as  destruction  of cancer  producing  pathogens,  oxidative  destruction  of xenoestrogens  and  other  carcinogens,  increasing  levels  of cancer  fighting  cytokines,  increasing  levels  of  superoxide dismutase  (SOD),  increasing  WBC  activity, decreasing lactic acid  levels by preventing its conversion  into  glucose through gluconeogenesis and direct destruction of  cancer cells through an overload of peroxide within the cancerous cells.

Treatment of SARS

Ozone is a naturally occurring energy-rich molecule with unique physicochemical and biological properties indicating a promising role in the therapy of SARS (severe acute respiratory syndrome), either as a monotherapy or, as an adjunct to standard treatment schedules. Owing to the excess energy contained within the O3molecule, it shows effectiveness across the entire genotype and subtype spectrum of SARS in comparison to the available organism-specific antiviral drugs.

Neurodegenerative diseases

Oxidative stress is one of the reasons for the progression of the neurodegenerative diseases like Alzheimer’s disease (AD), Parkinson’s disease(PD), Huntington’s disease (HD), and amyotrophic lateral sclerosis (ALS) which are related to the action of reactive oxygen species (ROS) in  the body. Research has found that the Nrf2/ARE {Nuclear factor (erythroid-derived 2) antioxidant response element (ARE)} pathway provide protection against neuro degeneration.  The ROS leads to mitochondrial dysfunction and neuro-inflammation while Nrf2 counteracts this action by activating the antioxidant response element (ARE) pathway.

Ozone therapy activates Nrf2 via moderate oxidative stress which results in protection against neurodegenerative diseases.

Age-Related Macular Degeneration (ARMD)

In the UK alone, some 250,000 patients affected by the ‘’dry’’(atrophic) form of ARMD are suitable for treatment with ozonated AHT[autohemotherapy], but all over the world there are more than 30 million people searching for a therapy.

Nonetheless, ophthalmologists can only prescribe anti-oxidants and zinc, which are minimally effective. Since 1995, almost 1,000 patients with the dry form of ARMD have been treated with ozonated AHT at the Siena (Italy) polyclinic and three- quarters have shown an improvement of one to two lines on the visual acuity chart.

Moreover, it is relevant that the disease does not progress during ozone therapy. This study emphasizes that ozone therapy is the only treatment able to dramatically improve the patient’s quality of life.

Treatment of backache and orthopedic disease

The problem of backache and orthopedic diseases has been successfully solved with ozone. In reality the use of ozone in hernial disk is a real breakthrough and, while the use of ozonated AHT is still regarded with suspicion, the application of ozone in low back pain is practically accepted world-wide.


At the present time, there are about eight simple methods of ozone therapy that are used in medical practice.

  1. Direct intra-arterial and intravenous application

An ozone/oxygen mixture is slowly injected into an artery or vein with a hypodermic syringe. This method is used primarily for arterial circulatory disorders. According to Gerard V. Sunnen, M.D., “Due to accidents produced by too rapid introduction of the gas mixture into the circulation, this technique is now rarely used” (1988).

  1. Rectal insufflations

First pioneered by Payr and Aubourg in the 1930’s, a mixture of ozone and oxygen is introduced through the rectum and absorbed into the body through the intestine. Used for a wide variety of health problems, this method is considered one of the safest. In a typical treatment for ulcerative colitis, for example, 75 micrograms of ozone per milliliter of oxygen are used (treatment begins with 50ml of oxygen which can be increased slowly to 500 ml per treatment). While administered under medical supervision in Germany, Russia and Cuba, a growing number of private individuals in the United States use this method for self-treatment for cancer, HIV-related problems and other diseases.

  1. Intramuscular injection

A small amount of an ozone and oxygen mixture (up to 10 ml) are injected into the patient (usually in the buttocks) like a normal injection would be. This method is commonly used to treat allergies and inflammatory diseases. Intramuscular injections are sometimes utilized as an adjunct to cancer therapies in Europe.

  1. Major and minor autohaemotherapy[AHT]

Used since the 1960’s, minor autohaemotherapy involves removing a small amount (usually 10 ml) of the patient’s blood from a vein with a hypodermic syringe. The blood is then treated with ozone and oxygen, and given back to the patient with an intramuscular injection. Thus the blood and ozone becomes a type of autovaccine given to the patient that is derived from their own cells, thus forming a unique vaccine that can be very specific and effective in treating the patient’s health problem.

Major autohaemotherapy calls for the removal of between 50-100 ml of the patient’s blood. Ozone and oxygen are then bubbled into the blood for several minutes, and then the ozonated blood is re-introduced into a vein. These methods have been used to treat a wide variety of health problems, including herpes, arthritis, cancer, heart disease and HIV-infection. It is probably the most commonly used type of ozone therapy today.

  1. Ozonated water

This method calls for ozone gas to be bubbled through water, and the water is used externally to bathe wounds, burns and slow-healing skin infections. It is also used as a disinfectant by dentists who perform dental surgery. In Russia, physicians are using ozonated water to irrigate body cavities during surgery. In both Russia and Cuba, ozonated water is used to treat a wide variety of intestinal and gynecological problems, including ulcerative colitis, duodenal ulcers, gastritis, diarrhea and vulvovaginitis (Proceedings of the First Iberolatinamerican Congress on Ozone Applications, 1990).

  1. Intra-articular injection

In this method, ozone gas is bubbled through water and the mixture is injected directly between the joints. It is used primarily by physicians in Germany, Russia and Cuba to treat arthritis, rheumatism and other joint diseases.

  1. Ozone bagging

This non-invasive method uses a specially made plastic bag that is placed around the area to be treated. An ozone/oxygen mixture is pumped into the bag and the mixture is absorbed into the body through the skin. Ozone bagging is primarily recommended for treating leg ulcers, gangrene, fungal infections, burns and slow-healing wounds.

  1. Ozonated oil

Used primarily to treat skin problems, ozone gas is added to olive oil and applied as a balm or salve for longterm, low-dose exposure.


1. All cases with Blood Coagulation Failure

2. Bleeding Organs

3. Thrombocytopenia

4. Ozone Allergy

5. Hemorrhagic or Apoplectic Stroke

6. Ozone Intolerance

NOTE: Ozone in low concentrations is known to produce a moderate hypo-coagulation effect, so all the drugs decreasing blood coagulation (anticoagulants, aspirin, etc.) are to be discontinued during the course of ozone therapy. In women the treatment course is to be broken up for menstrual periods.

Ozone IV Therapy References

  1. Gujral, Sarbjeet & Riyal, Poornima & Nand, Pratibha & Vashist, Neelam. (2013). Ozone therapy and its utilisation in current medical practices. Accessed 28th of January 2023
  2. Sagai, Masaru & Bocci, Velio. (2011). Mechanisms of Action Involved in Ozone Therapy: Is healing induced via a mild oxidative stress?. Medical gas research. 1. 29. 10.1186/2045-9912-1-29. Accessed 29th of January 2023.
  3. Lynch E. Ozone: The Revolution in Dentistry. Copenhagen, Quintessence books; 2004. [Google Scholar]
  4. Ozone therapy in practice. health manual Nizhny Novgorod, Russia, 2008 © Oleg V. Maslennikov, Claudia N. Kontorshchikova, Irina A. Gribkova.
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